Dr. Florian Rehfeldt (Göttingen): Microenvironment Mechanics Direct Stem Cell Cytoskeleton and Nuclear Morphology |
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| Startdatum/-zeit: | 04.06.2013 17:15 |
| Enddatum/-zeit: | 04.06.2013 19:00 |
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| Adresse: | Prof. Dr. Achim Paululat Koordination: apl. Prof. Dr. Merzendorfer Barbarastr. 11 49076 Osnabrück |
| Telefon: | 0541/969-3502 |
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| E-Mail: | merzendorfer@biologie.uni-osnabrueck.de |
| Homepage: | http://www.biologie.uni-osnabrueck.de/Fachbereich/?x=aaad,aczx,gn |
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| Adresse: | Biologiegebäude, Hörsaal 35/E01 Barbarastr. 11 49076 Osnabrück |
| Karte: | Bei Google Maps anzeigen |
| Beschreibung: | The mechanical properties of microenvironments in our body are very diverse and are as important to cells as biochemical cues. An especially striking experiment of this mechano-sensitivity demonstrated that systematic variation of the Young's modulus E of the substrate can direct the lineage differentiation of human mesenchymal stem cells (hMSCs). To elucidate the complex interplay of physical and biochemical mechanisms of cellular mechano-sensing, well-defined extracellular matrix (ECM) models are essential. While elastic substrates made of poly-acrylamide (PA) are widely in use, they have the potential drawback that the precursors are cytotoxic and therefore do not allow for 3D culture systems. Here, a novel biomimetic ECM model based on hyaluronic acid (HA) was successfully established that exhibits a widely tuneable and well-defined elasticity E, enables 2D and 3D cell culture and enables us to mimic a variety of distinct in vivo microenvironments. Quantitative analysis of the structure of acto-myosin fibers of hMSCs on elastic substrates with an order parameter S, reveals that the stress fiber morphology is an early morphological marker of mechano-guided differentiation and can be understood using a classical mechanics model. Furthermore, the cytoskeleton also dictates the shape of the nucleus and lends support to a direct mechanical matrix-myosin-nucleus pathway. |
| Kategorie: | SFB-Seminare (Biologie) |
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