How do multiprotein complexes responsible for processing immunological responses assemble inside cells? To address this challenge, researchers from the RTG2900 engineered high-density nanodot arrays using capillary nanostamping and tailored surface chemistry. By capturing transmembrane adaptors, nanoscale platforms were generated which locally trigger Myddosome assembly, enabling ultrastructural interrogation by super-resolution imaging at sub-nanometer resolution.
This work has been published in ACS Nano and selected as a Supplementary Cover:
A Modular Toolkit for Nanoscale Interrogation of Multiprotein Assemblies Inside Living Cells
Arthur Felker, Michael Philippi, Michael Holtmannspötter, Christoph Drees, Evelin Schäfer, Martin Steinhart, Rainer Kurre, Changjiang You, and Jacob Piehler
